DNA-based stable isotope probing identifies triclosan degraders in nitrification systems under different surfactants

Abstract

Three widely-used surfactants, rhamnolipid (RL), sophorolipid (SL) and sodium dodecyl benzene sulfonate (SDBS), were chosen to investigate their effects on the nitrification systems treating step-wised triclosan (TCS). Surfactants had little effects on nitrification. Surfactants could promote the desorption of TCS and enhance the TCS biodegradation in nitrification systems. And TCS biodegradation efficiencies obtained with RL, SL and SDBS were 1.25, 1.23 and 1.14 times higher than the control with 9.0 mg/L TCS, respectively. Illumina MiSeq sequencing showed that Amaricoccus could be resistant to TCS. And Amaricoccus, detected with RL, SL and SDBS, were more abundant than the control. DNA-based stable isotope probing assays revealed Amaricoccus was the major TCS degrader. And the addition of surfactants could obviously increase the diversity of active TCS degraders, especially for biosurfactants. It seems that the addition of surfactants showed positive effects for the nitrification systems treating TCS wastewater.