Abstract
Transcription factors bind to specific genomic sequences to control the flow of genetic information from DNA to mRNA. The preinitiation complex of these proteins includes TATA binding protein (TBP), which specifically recognizes the TATA box, a highly conserved region at the beginning of many genes. As many cancers result from the aberrant regulation of transcription factors, there is great interest, both academically and diagnostically, in a better understanding these binding events, and TBP is a core factor that can help guide the design of novel probes of transcription. Here, we report on the design and use of DNA‐based folding biosensors that are capable of quantitatively and sensitively detecting TBP using custom optical and electrochemical probes that are activated by TBP's intrinsic DNA‐binding activity. These sensors will ultimately allow us to investigate the role that protein‐protein interactions and inhibitory drugs have on gene expression.
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