Abstract
Bacteria derived from swamp sediments and aquaculture ponds potential to be probiotics. The study aims to determine the sequence of 16S r RNA gene of isolate of probiotics candidate bacteria from sediment of swamp, to determine the phylogenetic tree between the bacterial species from isolates and Gene Bank data central for their potential as probiotic. The samples of bacteria resulted from pure isolation selected from the sediment of pond cultivation and swamp waters at Lebung Karangan Reservation, Ogan Ilir Regency, Indralaya, South Sumatra. This study was started from cultivating bacteria, extracting DNA of bacteria, amplification 16S r RNA genes by PCR, running electrophoresis, and sequencing the amplicon for determining DNA barcodes of bacteria from sediment of swamp and rearing pond. The result of BLAST analysis showed that KA isolate had the highest similarity 97% withStreptomyces sp.Hjorring101 from Denmark and RA isolate had the highest similarity 98% withStreptomyces sp. BD99 from Pakistan. KE isolate had the highest similarity 99% withBacillus subtilisCESi5 from Japan and RE isolate had the highest similarity 93% withBacillus sp.2bFR from Manado. All of isolates were bacteria potentially as swamp aquaculture probiotics.
Highlights
The swamp aquaculture had been developing at South Sumatra, Indonesia
The problem can be approached by specific swamp probiotic for increasing water quality and feed efficiency of swamp fish aquaculture
The isolates of bacteria were 4 pure isolates consisting of 2 eubacteria isolates and 2 actinomycetes isolates, each bacteria derived from sediments of swamp waters and aquaculture ponds
Summary
The swamp aquaculture had been developing at South Sumatra, Indonesia. Swamp land can be optimized based on its water level. The problem can be approached by specific swamp probiotic for increasing water quality and feed efficiency of swamp fish aquaculture. To ascertain the species of bacteria that are thought to be potential candidates for probiotics, there is a need for genetic identification that shows the results of specific identification. For this reason, it is necessary to know the molecular characteristics of probiotic bacteria using 16S rRNA gene sequencing. The purpose of this study were to know the feasibility of bacterial isolates that have the potential as probiotics from swamp sediments and catfish culture ponds based on their proximity to the probiotic genus by phylogenetic tree approach
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