Abstract
Orchids comprise the second-largest family of flowering plants in the world. Most of the Indian orchids are horticulturally and medicinally important because of having exotic flowers and many important phytochemicals and secondary metabolites. Unfortunately, endemic Indian orchids are rare, threatened, and listed in Appendix 1 and 2 of CITES. Hence, having a reliable method for identifying orchids is an immediate need to save them. DNA barcoding is a popular and useful technology for quickly and accurately identifying plant species. The present survey looked to find the best suitable research papers on DNA barcoding of Indian orchids using standard search engines. We explored studies published between 2003 and 2023. A total of 545 appropriate studies were systematically analyzed. We found seven relevant studies on the barcoding of Indian orchids, where researchers deliberated rpoC1, rpoB, rbcL, and matK from the chloroplast genome, trnH-psbA, an intergeneric spacer, and nrITS from the nuclear genome to find best candidate barcode for orchids in India. Our survey concludes that ITS is the most effective candidate barcode for the identification of Indian orchids among single-locus barcoding genes. The matK in combination with ITS is best among multilocus barcodes for accurate identification of orchid species. . KEYWORDS :Coelogyne, Dendrobium, ITS, matK, Paphiopedilum
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