Abstract
Odonates (Insecta: Odonata) are important insects in the food chains of freshwater environments around the world, being used as a model species for areas of behavior and analysis of environmental quality. In Brazil, especially in the Northeastern region, both knowledge about the distribution and molecular information of odonate species found in the two main biomes of the region is still limited. Aiming to improve these issues, here, we carried out an Odonata survey in two locations and built a DNA barcode database for species from the state of Paraíba. In total, 15 first records were reported for this Brazilian state and 142 specimens from 27 genera and 45 species had their ‘Folmer’ cytochrome c oxidase subunit I (COI) fragment evaluated. The database we generated includes data for 70% of the Odonata species found in Paraíba state. For 16 species, this is the first DNA barcode available in public sequence repositories. Our results demonstrate that using the COI in the regional scale can help identify and delimit those evaluated. Eight species (17%) showed a low percentage of differentiation (<2%) compared to other species currently deposited in the GenBank or BOLD System; nevertheless, we present morphological traits that reaffirm our identifications. Barcode data provide new insights into Neotropical diversity and deliver basic information for taxonomic analyses.
Highlights
Sequences is the first publication of barcoding for the. This library of DNA sequences is the first publication of DNA barcoding for the 16 species in public sequence repositories: Acanthagrion gracile (Rambur, 1842), A. januaria, Ep. metallica, E. avittata Borror, 1942, E. leticia, E. cf. fervida, E. basalis, E. cf. unimaculata (De Geer, 1773), M. griseofrons Calvert, 1909, Mecistogaster kesselringi Soldati and Machado, 2019, Metaleptobasis bicornis (Selys, 1877), N. berlai, O. flavopicta, Progomphus dorsopallidus
In addition to demonstrating that the two pairs of primers used in this study are capable of amplifying the c oxidase subunit I (COI) fragment in the two suborders present in the Neotropical region, this study shows that it is important to consider the usefulness of this marker to discriminate between different species in terms of their DNA barcode and metabarcoding studies
Our results showed that the analyzed specimens of A. januaria from Paraíba state are
Summary
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. Odonates (Insecta: Odonata) are a group of fascinating insects that can be found all over the world (except for Antarctica) [1]. They have been used as model animals in several areas, such as behavioral studies and the analysis of environmental quality (e.g., [2–4]). This group plays an important role in the trophic network of freshwater environments, both as an efficient predator of invertebrates and as a prey for several vertebrates [5]. Scientists have increasingly discovered new species, which has improved the taxonomic knowledge of odonates in the Neotropical realm; information on their taxonomy (‘Linnean’ shortfall), distribution (‘Wallacean’ shortfall) and genetic diversity remains scarce (see [6,7])
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
