DNA Barcoding and Genetic Structure Analysis of Deep-Sea Notacanthiform Fishes

Abstract

Event Abstract Back to Event DNA Barcoding and Genetic Structure Analysis of Deep-Sea Notacanthiform Fishes David Barros-García1*, Rafael Bañon1, Juan Carlos Arronte2, Lourdes Fernandez3, Ramón García3 and Alejandro De Carlos1 1 University of Vigo, Spain 2 Spanish Institute of Oceanography, Spain 3 Spanish Institute of Oceanography, Spain Notacanthiformes Goodrich, 1909 is an order of deep-sea, benthopelagic or benthic fishes distributed from the continental slope to the abyssal plain, at a depth of between 125 and 4,900 m, but mostly occurring at depths of 450-2,500 m. They are characterized by an eel-like body, a snout projecting conspicuously beyond the mouth, large connective tissue nodules inserted between the pterygoid arch and maxilla and pelvic fin webs joined in the ventral midline. Fishes from this order were classified applying DNA barcoding. Cytochrome c oxidase subunit I (COI) sequences belonging to new North Atlantic specimens and already deposited BOLD public records were used. The specimens from the two families of the order, Halosauridae (halosaurs) and Notacanthidae (spiny eels), formed separated monophyletic clades in neighbor-joining trees and the sequences clustered as coherent species. Nine out of 16 species of Halosauridae and 9 out of 10 species of Notacanthidae were represented including 166 sequences of which 96% were successfully identified. The DNA barcode of the rare species Lipogenys gillii was obtained for the first time ever. The DNA barcode was further tested by exploring the genetic structure and historical demography of four species of notacanthiforms from five sample locations of the North Atlantic and South West Pacific. Neutrality tests, mismatch distribution and haplotype networks analyses pointed to a past bottleneck episode followed by a fast demographic expansion for all the samples. The genetic structure of the abyssal halosaur Halosauropsis macrochir showed no significant differences between the North Atlantic and South West Pacific samples. DNA barcoding was successful in validating field identifications and assigning species names to sequences of notacanthiforms worldwide. These results constitute a first example of high connectivity and gene flow in this group of deep-sea fish species. The historical demography suggests population variations that may be due to past glaciation events. References Barros-García, D., Bañón, R., Arronte, J. C., Fernández-Peralta, L., García, R., & de Carlos, A. (2015). DNA barcoding of deep-water notacanthiform fishes (Teleostei, Elopomorpha). Zoologica Scripta. doi:10.1111/zsc.12154 Keywords: DNA barcoding, fish, Population Genetics, systematics, Evolution, Molecular Conference: XV European Congress of Ichthyology, Porto, Portugal, 7 Sep - 11 Sep, 2015. Presentation Type: Oral Presentation Topic: Phylogeny, Systematics and Genetics Citation: Barros-García D, Bañon R, Arronte J, Fernandez L, García R and De Carlos A (2015). DNA Barcoding and Genetic Structure Analysis of Deep-Sea Notacanthiform Fishes. Front. Mar. Sci. Conference Abstract: XV European Congress of Ichthyology. doi: 10.3389/conf.FMARS.2015.03.00179 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 30 Nov 2015; Published Online: 01 Dec 2015. * Correspondence: Mr. David Barros-García, University of Vigo, Vigo, Spain, Davbarros@uvigo.es Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract Supplemental Data The Authors in Frontiers David Barros-García Rafael Bañon Juan Carlos Arronte Lourdes Fernandez Ramón García Alejandro De Carlos Google David Barros-García Rafael Bañon Juan Carlos Arronte Lourdes Fernandez Ramón García Alejandro De Carlos Google Scholar David Barros-García Rafael Bañon Juan Carlos Arronte Lourdes Fernandez Ramón García Alejandro De Carlos PubMed David Barros-García Rafael Bañon Juan Carlos Arronte Lourdes Fernandez Ramón García Alejandro De Carlos Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.