DNA-barcoding and a new data about the karyotype of Myotis petax (Chiroptera, Vespertilionidae) in the Russian Far East

Denis V Kazakov,Irina N Sheremetyeva,Uliana V Gorobeyko,Valentin Yu Guskov,Irina V Kartavtseva

doi:10.3897/compcytogen.v14.i4.54955

Abstract

The DNA-barcoding and chromosomal study of the eastern water bat, Myotis petax Hollister, 1912, from the earlier unexplored localities in the Russian Far East are carried out. The COI barcoding obtained for 18 from a total of 19 individuals captured in five localities in the Russian Far East showed the low nucleotide variability with the prevalence of the central, the most abundant haplotype. The chromosomal characteristics of eight M. petax specimens (2n = 44, NFa = 52) in the Russian Far East are clarified. The number and localization of NOR in karyotype of M. petax is described at the first time and differ from distributional patterns of NOR in the sibling species M. daubentonii Kuhl, 1819 that can be used as diagnostic feature. The considerable intraspecific variability in the distribution of heterochromatin material revealed is not typical of the genus Myotis, but it has been found in other species of the family Vespertilionidae.

Highlights

The eastern water bat, Myotis petax Hollister, 1912, is a common Eastern Palaearctic bat species
Starting from Ognev (1928) and until the early 2000s, M. petax had been considered as part of the widespread polytypic species Myotis daubentonii Kuhl, 1819 which had included about 3 to 6 subspecies according to various estimates (Kuzaykin 1950; Gromov 1963; Tiunov 1984, 1997; Yoshiyuki 1989; Bogdanowicz 1994; Koopman 1994)
A total of 6 specimens of M. daubentonii and 7 specimens of M. petax were examined by molecular method (Matveev et al 2005). It was shown by the molecular studies based on cyt b and ND1 sequences that M. petax is closer related to M. macrodactylus (Temminck, 1840), M. pilosus Peters, 1869 and M. fimbriatus Peters, 1871 than M. daubentonii

Summary

Introduction

The eastern water bat, Myotis petax Hollister, 1912, is a common Eastern Palaearctic bat species. The range of M. petax includes the near-water habitats throughout forest, forest-steppe and steppe zones from Western Siberia to the Russian Far East (including Sakhalin and the Kuril Islands) and, outside of Russia – in northern Mongolia, NE China, Korea and Japan (Kruskop 2012). It was first described as a distinct species from the village Kosh-Agach in the Altai Mountains (Hollister 1912). A total of 6 specimens of M. daubentonii and 7 specimens of M. petax (including only one bat from the Far East) were examined by molecular method (Matveev et al 2005). 1823 (Kawai et al 2002, 2003; Ruedi et al 2013; Ruedi et al 2015)

Methods

Results

Conclusion