Abstract
In this article, carbon dots (CDs) were synthesized via hydrothermal treatment of citric acid and ethylenediamine, and modified on the surface to generate HOOC-CDs. 3′-NH2-terminated capture ssDNA (DNA1) combined with HOOC-CDs to prepare CDs-DNA1 complex through carbodiimide bonding. Due to the specific DNA complementary combination of DNA1 and DNA2, CDs-DNA1 combined with target ssDNA (DNA2) labelled with 5-fluorouracil (5Fu) at the 5′-end to generate CDs-5Fu DNA assembly. After loading of glucosamine (Glu) into double-strands complementary DNA, CDs-5Fu-Glu hybrid as a versatile carrier was synthesized. DNA2 in the hybrid served as an aptamer of alpha fetal protein (AFP) and specifically combined with AFP, which could trigger the unwinding of CDs-DNA1 and 5Fu-DNA2, and simultaneous release of 5Fu and Glu. In the hybrid, 5Fu induced room-temperature phosphorescence (RTP) quenching of CDs owing to photoinduced electron transfer from CDs to 5Fu. The addition of ATP resulted in the separation (drug release) of 5Fu and Glu from CDs, accompanied by RTP recovery of CDs. Experimental results verified that CDs-5Fu-Glu hybrid could be developed towards a novel RTP turn-on probe for AFP detection and an advanced carrier for AFP-triggered simultaneous release of 5Fu and Glu.
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