Abstract
High altitude exposure known as hypobaric hypoxia (HH) is associated with an oxidative metabolism that could increase the reactive oxygen species production. Sperm cells are particularly susceptible to oxidative damage in lipids and DNA, affecting their functions and fertilization potential. The aim of this study was to determine the DNA oxidative damage and lipoperoxidation (LPO) induced by the exposure to continuous chronic HH (CCHH) and intermittent chronic HH (ICHH) in rat epididymal sperm cells. Using western blot assay, the expression of the 8-oxoguanine DNA glycosylase 1 (Ogg1) was used to evaluate the genomic and mitochondrial DNA reparation capacity against the oxidative lesion 8-oxo guanine (8-oxoG), whereas the presence of the biomarker 8-oxoG in sperms was determined through immunofluorescence. LPO levels were obtained by malondialdehyde (MDA) measurement. The results showed a decrease in the Ogg1 mitochondrial and nuclear isoform expression in both treatments of HH, increase of the 8-oxoG lesions in the sperm anatomy and also an increase in the MDA content in ICHH. However, these values kept constant at the end of the exposure CCHH. In conclusion, sperm susceptibility increases in HH, because DNA repair enzymatic machinery is limited and conduces to an accumulation of oxidative DNA lesions and sperm LPO increases specially in intermittent exposure. By grant: INNOVA CORFO-Chile 06FCO11BC-118
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