DMAP-catalysed synthesis, antibacterial activity evaluation, cytotoxicity and docking studies of some heterocyclic molecules bearing sulfonamide moiety

Abstract

DMAP has been shown to be a highly efficient nucleophilic catalyst when compared to triethylamine and pyridine using acetonitrile as solvent for the synthesis of a series of novel N - heterocyclic sulfonamide derivatives. The influence of the reaction parameters, like choice of solvent, catalyst, amount of catalyst and reaction time on product yield has been studied. Antibacterial screening involving a range of sulfonamide analogues as new peptide deformylase (PDF) inhibitors have been focused. The molecules show significant antibacterial activity (MIC value 6.2 − 3.1 µg/mL) against B. subtilis , S. pyrogenes , P. vulgaris and P. mirabilis . Potential in silico docking studies have been in conjugation with in vitro antibacterial results. Molecular docking of all compounds with PDF enzyme (PDB code: 1G2A) explain how certain moieties play significant roles in increasing the binding interactions and stabilizing the protein-ligand complexes. The compounds also have confirmed low extent of cytotoxicity when tested on HEL and HeLa cell lines.