Abstract
BackgroundObesity is a metabolic imbalance characterized by excessive deposition of white fat. The browning of white fat can effectively treat obesity and related diseases. Although Dlgap1 (Discs, Large (Drosophila) Homolog-Associated Protein 1) is suspected to have an effect on this process, no empirical evidence is available.MethodsTo understand the role of Dlgap1, we cultured white and brown fat cells, then performed overexpression and knockout experiments.ResultsWe found that Dlgap1 overexpression in brown adipocytes inhibits brown-fat-related gene expression, promotes white-fat-related genes, while also increasing brown-adipocyte proliferation and apoptosis. However, the gene overexpression has no effect on brown adipocyte maturation. Knocking out Dlgap1 in white fat cells promotes the expression and inhibition of brown-fat-related and white-fat-related genes, respectively. Additionally, the knockout inhibits white fat cell proliferation and apoptosis, while also promoting their maturation.ConclusionsDlgap1 negatively regulates the browning of white adipocytes by influencing cell proliferation and apoptosis.
Highlights
Obesity is a metabolic imbalance characterized by excessive deposition of white fat
After 10 d of culturing experimentally isolated adipocytes, we observed that the white fat cells were 80% confluent, while the brown fat cells were only 40% confluent, indicating a significantly slower growth rate of the latter
We found that the white-fat marker genes Asc1, Leptin, and Adipoq [32] were 3.03, 9.33, and 207.03 times higher in white adipocytes than in brown adipocytes, respectively
Summary
Obesity is a metabolic imbalance characterized by excessive deposition of white fat. The browning of white fat can effectively treat obesity and related diseases. Results: We found that Dlgap overexpression in brown adipocytes inhibits brown-fat-related gene expression, promotes white-fat-related genes, while increasing brown-adipocyte proliferation and apoptosis. Knocking out Dlgap in white fat cells promotes the expression and inhibition of brown-fat-related and white-fat-related genes, respectively. The knockout inhibits white fat cell proliferation and apoptosis, while promoting their maturation. Conclusions: Dlgap negatively regulates the browning of white adipocytes by influencing cell proliferation and apoptosis. This article verifies that Dlgap expression is significantly different in white and brown fat and negatively regulates the browning of white adipocytes by influencing cell proliferation and apoptosis
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
