Abstract
BackgroundLong non-coding RNAs (lncRNAs) have been reported to be biological regulators in hepatocellular carcinoma (HCC). DLG1 antisense RNA 1 (DLG1-AS1) has been found to be up-regulated in cervical cancer. However, its function and underlying mechanism in HCC remains unknown.MethodsDLG1-AS1 expression was assessed in HCC cells and normal cell by RT-qPCR. Luciferase reporter assay, RNA pull down assay and RIP assay were used to demonstrate the interaction between DLG1-AS1 and miR-497-5p.ResultsDLG1-AS1 was highly expressed in HCC cells. Silencing of DLG1-AS1 led to the inhibition of HCC cell growth and migration. Besides, MYC induced the transcriptional activation of DLG1-AS1. MYC could facilitate HCC cellular processes by up-regulating DLG1-AS1. MiR-497-5p could interact with DLG1-AS1 in HCC cells. Down-regulation of miR-497-5p could reverse the impacts of DLG1-AS1 silencing on HCC cells. SSRP1 expression could be positively regulated by DLG1-AS1 but was negatively regulated by miR-497-5p. Knockdown of DLG1-AS1 suppressed tumor growth in nude mice.ConclusionsDLG1-AS1 is activated by MYC and functions as an oncogene in HCC via miR-497-5p/SSRP1 axis.
Highlights
Long non-coding RNAs have been reported to be biological regulators in hepatocellular carcinoma (HCC)
DLG1‐AS1 promotes the growth and migration of HCC cells Data of RT-qPCR revealed that DLG1-AS1 was expressed higher in HCC cell lines (MHCC97-L, Huh-7, Hep3B and HCCLM3) than that in the normal human liver immortalized cell line (THLE-2) (Fig. 1a)
Since Hep3B and HCCLM3 possessed highest expression of DLG1-AS1 in HCC cells, they were used in the following assays
Summary
Long non-coding RNAs (lncRNAs) have been reported to be biological regulators in hepatocellular carcinoma (HCC). DLG1 antisense RNA 1 (DLG1-AS1) has been found to be up-regulated in cervical cancer. Hepatocellular carcinoma (HCC) is one of the most aggressive and fatal cancers among human beings all over the world, which is hard to be cured due to metastasis [1]. Long non-coding RNAs (lncRNAs) belong to noncoding RNAs, which have no or limited protein-coding capacities [5]. LncRNA GHET1 is a biomarker in HCC and accelerates tumor progression by targeting KLF2 [9]. DLG1 antisense RNA 1 (DLG1-AS1) has been identified as a novel oncogenic lncRNA in cervical cancer [11]. It has been proven to be a tumor promoter in breast cancer by negatively regulating miR203 [12]. The role of DLG1-AS1 in HCC has not been well explained
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