Abstract
Microbial lipids derived from lignocellulosic biomass are attractive feedstocks for biodiesel production. However, toxic degradation products generated by pretreatment greatly inhibited lipid fermentation. Herein, two pretreatment methods, i.e. densifying lignocellulosic biomass with sulfuric acid (DLC(sa)) and densifying lignocellulosic biomass with sulfuric acid followed by autoclave (DLCA(sa)), were developed on rice straw (RS) for lipid production. The conditions of DLCA(sa) pretreatment, including sulfuric acid dosage, dry biomass loading and autoclave time, were optimized by response surface methodology. High fermentable sugar yields with low inhibitor concentrations were obtained on DLC(sa) pretreated RS (DLC(sa)-RS) and DLCA(sa) pretreated RS (DLCA(sa)-RS) via enzymatic hydrolysis at a high solid loading of 30 wt%. Lipid titers of as high as 28.9 g/L and 31.0 g/L, with lipid yields of 0.22 g/g and 0.24 g/g consumed sugar, were achieved when RS was pretreated by DLC(sa) and DLCA(sa), respectively. This study demonstrated efficient microbial lipid production from lignocellulosic biomass without washing or detoxification steps.
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