DLBS3233 increases glucose uptake by mediating upregulation of PPARγ and PPARδ expression

Abstract

DLBS3233 is a standardized extract combination containing Lagerstroemia speciosa and Cinnamomum burmannii. The effect on glucose uptake, adiponectin secretion, and insulin signaling of DLBS3233 (1–5 μg/ml) was examined in this study. 3T3 Swiss albino preadipocytes and adipocytes were used to investigate gene expressions which were detected using reverse transcription PCR method. DLBS3233 was found to enhance the expression of genes associated with increased insulin signaling and sensitivity such as PPARγ and PPARδ. In addition, the expressions of PI3 kinase, Akt, as well as GLUT4 genes were seen to be increased. This extract combination also increased glucose uptake, increased adiponectin secretion, and decreased resistin secretion significantly relative to control cells. Moreover, DLBS3233 administered in insulin resistant Wistar strain rats ( Rattus norvegicus strain Wistar), showed a property to control blood glucose, insulin and cholesterol levels. Therefore, DLBS3233 is a promising combination of herbal extracts which conveys medical utilities in term of type II diabetes treatment, and, possibly also in diabetes disease prevention.