Division of Labor Between Two Actin Nucleators-the Formin FH1 and the ARP2/3 Complex-in Arabidopsis Epidermal Cell Morphogenesis.

Petra Cifrová,Viktor Žárský,Kateřina Schwarzerová,Eva Kollárová,Jan Martinek,Denisa Oulehlová,Amparo Rosero,Fatima Cvrčková

doi:10.3389/fpls.2020.00148

Abstract

The ARP2/3 complex and formins are the only known plant actin nucleators. Besides their actin-related functions, both systems also modulate microtubule organization and dynamics. Loss of the main housekeeping Arabidopsis thaliana Class I membrane-targeted formin FH1 (At3g25500) is known to increase cotyledon pavement cell lobing, while mutations affecting ARP2/3 subunits exhibit an opposite effect. Here we examine the role of FH1 and the ARP2/3 complex subunit ARPC5 (At4g01710) in epidermal cell morphogenesis with focus on pavement cells and trichomes using a model system of single fh1 and arpc5, as well as double fh1 arpc5 mutants. While cotyledon pavement cell shape in double mutants mostly resembled single arpc5 mutants, analysis of true leaf epidermal morphology, as well as actin and microtubule organization and dynamics, revealed a more complex relationship between the two systems and similar, rather than antagonistic, effects on some parameters. Both fh1 and arpc5 mutations increased actin network density and increased cell shape complexity in pavement cells and trichomes of first true leaves, in contrast to cotyledons. Thus, while the two actin nucleation systems have complementary roles in some aspects of cell morphogenesis in cotyledon pavement cells, they may act in parallel in other cell types and developmental stages.

Highlights

IntroductionThe shape of plant cells (and subsequently organs and tissues) is mainly controlled by orchestrated action of microfilaments, microtubules, and membrane trafficking in the cortical cytoplasm, resulting in spatially and temporally controlled cell growth and cell wall synthesis
The shape of plant cells is mainly controlled by orchestrated action of microfilaments, microtubules, and membrane trafficking in the cortical cytoplasm, resulting in spatially and temporally controlled cell growth and cell wall synthesis
The impact of simultaneous perturbation of both actin nucleation systems on Arabidopsis epidermal cells shaping was investigated for the first time, with focus on interdigitated epidermal pavement cells and trichomes

Summary

Introduction

The shape of plant cells (and subsequently organs and tissues) is mainly controlled by orchestrated action of microfilaments, microtubules, and membrane trafficking in the cortical cytoplasm, resulting in spatially and temporally controlled cell growth and cell wall synthesis. The cortical cytoplasm controls localization of membrane proteins including auxin transporters, contributing to cell differentiation and affecting tissue- and organ-scale developmental processes (e.g., Žárský et al, 2009; Qi and Greb, 2017). Angiosperms have two clades of formins consisting of multiple paralogs, with over 20 genes in Arabidopsis (Grunt et al, 2008) Besides their actinrelated roles, formins contribute to the coordination between microfilaments and microtubules (Bartolini and Gundersen, 2010; Wang et al, 2012; Henty-Ridilla et al, 2016). Direct or interactor-mediated membrane association, or role in endomembrane organization, is documented for some opisthokont formins lacking membrane insertion motifs (reviewed in Cvrčková, 2013; see, e.g., Copeland et al, 2016)

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