Diversity of the Polish isolates of bean yellow mosaic virus (BYMV) isolated from broad bean

Abstract

Bean yellow mosaic virus (BYMV) is one of the most widespread and damaging viruses of cultivated legumes in the world. In order to characterise BYMV isolates detected in ten broad bean plants in Poland, total nucleic acids were isolated, a fragment of the gene encoding the polyprotein was amplified by RT-PCR and then sequenced. The sequences of the coat protein (CP) gene of the three Polish isolates (E1, E2, E3) were compared with 33 sequences retrieved from the GenBank database. The sequences of the tested isolates showed 81,8–99,8% identity at the nucleotide level and 84,9–99,6% at the amino acid level with other BYMV isolates. The analysis performer based on CP sequences confirmed the genetic diversity of the BYMV population and revealed the presence of three phylogenetic groups. The Polish isolates were classified into I (E1 and E2) and III (E3) group.