Diversity of the killer cell immunoglobulin‐like receptor gene KIR2DS4 in the Chinese population

Abstract

Human killer cell immunoglobulin-like receptors are a subfamily of the immunoglobulin superfamily, which map to the leukocyte receptor complex on chromosome 19. Here, we established polymerase chain reaction-sequence-based typing (PCR-SBT) procedures to identify alleles of the KIR2DS4 gene. The method was designed around the specific amplification of exons 4-5 of the KIR2DS4 gene. Genomic DNA from 105 healthy unrelated Chinese Han individuals were typed for the KIR2DS4 alleles. Each sample was assigned the KIR2DS4 alleles combination, consistent with the pairwise combinations of sequences of all the known KIR2DS4 alleles. We observed eleven different genotypes and four KIR2DS4 alleles in the population, with the KIR2DS4*00101 having the highest frequency, 0.576, and also confirmed the new KIR2DS4*007 allele. Our data demonstrated that the established PCR-SBT method for the KIR2DS4 allele typing was reliable.