Diversity of Ni growth response and accumulation in Central-Eastern Mediterranean Odontarrhena (Brassicaceae) populations on and off serpentine sites

Abstract

Nickel response and accumulation were analyzed in 12 populations from seven Central-Eastern Mediterranean Odontarrhena taxa on and off serpentine sites to test the presence of metal-induced growth stimulation and its relationship with tolerance and plant Ni concentration. Seedlings were cultivated in hydroponics with increasing NiSO4 concentrations to obtain dose-response curves and to evaluate Ni levels in roots and shoots. In all the accessions, a metal stimulatory effect on growth was present in the low-dose zone and significantly fitted the Brain-Cousens hormetic model. Accessions showed broad variation in tolerance, with the most tolerant plants requiring the highest Ni concentrations in the culture medium for optimal growth. Significant differences were also detected in plant Ni concentrations and a positive relationship was found between tolerance and accumulation. The serpentine and non-serpentine populations of O. chalcidica were similarly capable of hyperaccumulation, suggesting this ability to a be a species-wide trait. In the case of O. muralis, a species that preferentially avoids serpentine soils, the two populations from non-serpentine sites showed the same Ni-enhanced growth of the serpentine accessions of the other species investigated here, but only at the lowest concentration, and reached shoot Ni amounts approaching the Ni hyperaccumulation threshold. Therefore, in Odontarrhena the capacity to tolerate and accumulate Ni could be considered a specieswide trait in all the serpentine taxa, both endemic and facultative. Regarding the metal concentration showed by the accessions in nature, neither Ni levels in the field-collected plants nor those in their natural soils of origin were related to Ni tolerance. On the other hand, Ni accumulation capacity appeared as the main driving factor for the metal concentration in the plants in their native habitats.