Diversity of Microbial Communities Associated with Epilithic Macroalgae in Different Coral Reef Conditions and Damselfish Territories of the Gulf of Thailand

Abstract

Reef degradation and algae-farming fish alter the structures and compositions of coral-algal-microbial communities. We collected epilithic macroalgae in different reef conditions and damselfish territories. The microbial communities were characterized by subjecting the V3-V4 hypervariable region of the 16S rRNA gene to amplicon sequencing. Metagenomic analysis revealed 2 domains, 51 phyla, 112 orders, and 238 families and the dominance of Proteobacteria and Bacteroidota in both fair and degraded reefs inside and outside territories. Chloroflexi on the degraded reef was dominant and its proportion was almost two and a half times compared to the fair reef, whereas Cyanobacteria was low on the degraded reef. Bacteroidota was dominant on the fair reef, whereas Actinobacteriota was scarce on this reef. For the damselfish territories, Chloroflexi was dominant inside the territory, whereas Bacteroidetes were found outside the territory. Differences in the microbial species diversity and richness were not apparent between all sites; however, species evenness was higher on the degraded reef condition and lower outside the territory. Important potential pathogens of reef organisms, such as Vibrio, Photobacterium, and Phormidium, were found on the degraded reef areas inside the damselfish territory. The farming behaviors of damselfish influenced microbial communities by changing the epilithic algal matrix that harbors many microbial communities. This study provides useful information on microbial biota in coral reef habitats which is further applicable to reef conservation and coastal management.