Abstract
AbstractIsozymes (esterase and malate dehydrogenase), SCAR and RAPD-PCR were studied in 15 populations of three races of Meloidogyne exigua collected in coffee-producing areas in Brazil, Bolivia and Costa Rica and one population from rubber tree plantations in Brazil. This study revealed four esterase phenotypes (E1, E2, E2a, E3) and three malate dehydrogenase phenotypes (N1, N1a, N2) for M. exigua populations. The most common multi-enzyme phenotype was E2N1. The enzymatic phenotypes do not separate M. exigua races. Sixteen populations of M. exigua were tested in Multiplex PCR using SCAR primers ex-D15F/R that allowed the identification of all M. exigua populations. Phylogenetic analyses showed high intraspecific polymorphism (25.9-59.6%) for all M. exigua studied. However, all populations clustered together with 100% bootstrap support, thereby demonstrating the consistency of species identification. In general, no correlation was found between enzymatic profile, race and genetic polymorphism of the studied populations.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
