Diversity of Listeria monocytogenes Strains Isolated from Food Products in the Central European Part of Russia in 2000-2005 and 2019-2020.

Ekaterina K Psareva,Nadezda A Gladkova,Eugene A Potemkin,Elena A Liskova,Irina V Razheva,Denis V Kolbasov,Maria A Grudistova,Yulia K Yushina,Olga L Voronina,Pavel A Zhurilov,Elena V Sokolova,Pavel A Andriyanov,Svetlana A Ermolaeva

doi:10.3390/foods10112790

Ekaterina K Psareva, Nadezda A Gladkova + Show 11 more

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https://doi.org/10.3390/foods10112790

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Abstract

Totally, 45 L. monocytogenes strains isolated from meat, poultry, dairy, and fish products in the Central European part of Russia in 2001–2005 and 2019–2020 were typed using a combined MLST and internalin profile (IP) scheme. Strains belonged to 14 clonal complexes (CCs) of the phylogenetic lineages I and II. Almost half of the strains (20 of 45) belonged to six CCs previously recognized as epidemic clones (ECs). ECI and ECV strains were isolated during both studied periods, and ECII, ECIV, ECVI, and ECVII strains were isolated in 2001–2005, but not in 2019–2020. ECI, ECIV, ECV, and ECVII strains were isolated from products of animal origin. ECII and ECVI were isolated from fish. Testing of invasion efficiencies of 10 strains isolated in different years and from different sources and belonging to distinct CCs revealed a statistically significant difference between phylogenetic lineage I and II strains but not between ECs and non-EC CCs or strains differing by year and source of isolation. Strains isolated in 2001–2005 were characterized by higher phylogenetic diversity and greater presentation of ECs and CCs non-typical for natural and anthropogenic environments of the European part of Russia comparatively to isolates obtained in 2019–2020.Closing of the Russian market in 2019–2020 for imported food might be responsible for these differences.

Highlights

The Gram-positive bacteria Listeria monocytogenes causes a severe foodborne disease, listeriosis
The 45 strains were prescribed to 14 sequence types (STs) belonging to distinct clonal complexes (CCs) (Table S1)
Prescription of clonal complexes to phylogenetic lineages according to the MLST database BIGSdb-Lm https://bigsdb.pasteur.fr/listeria/listeria.html showed in 2001–2005 and 2019–2020

Summary

Introduction

The Gram-positive bacteria Listeria monocytogenes causes a severe foodborne disease, listeriosis. Listeriosis has harsh clinical manifestations and high mortality among groups of risk that include elderly and/or immunocompromised persons, pregnant women, and newborns [1,2]. The most serious manifestations of listeriosis are disorders of the central nervous system and miscarriagesin pregnant women [3]. There are reports of listeriosis outbreaks, both among patients and after eating of listeria infected food products [4,5]. L. monocytogenes entering the intestine with contaminated food is able to cross the intestinal epithelial barrier and to colonize lamina propria, from where it spreads to internal organs, primarily to the liver and spleen. If the infection is not controlled at this stage, secondary bacteremia develops and bacteria can cross thebrain–

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