Diversity of Halomonas native species to have nitrifying capability isolated from Litopenaeus vannamei farm

Abstract

The objective of the research was to identify the species of the genus Halomonas, with nitrifying capacity, isolated from sediments of Litopenaeus vannamei farms from shrimp farms in Tumbes-Peru. From the sediment of 3 prawn farms at harvest time, a representative sample was obtained, to which a pre-enrichment was carried out in Marine Broth for 48 hours, then it was cultured in Marine Agar, until the observation of colonies characteristic of the genus Halomonas. The cultures obtained were identified phenotypically at the species level, to then evaluate their nitrifying capacity according to the Griess-llosvay methodology. As results, the isolation of 5 bacterial cultures was obtained, two identified as H. sulfidaeris and 3 as H. salina; All cultures showed the ability to reduce nitrate to nitrite by eliminating ammonium from the medium. It is concluded that in the sediment of Litopenaeus vannamei breeding sites located in Tumbes-Peru, the species H. sulfidaeris and H. salina are present, with nitrifying capacity, which allow the elimination of ammonium, which is developed as an effective option for the saline ammonium wastewater treatment.