Abstract
Diversity of fumarate reducing (dissimilating) bacteria in the bovine rumen was analyzed by both culture dependent and independent methodologies. A total of 39 strains were isolated by using three different media and belonged to three different phyla ( Proteobacteria, Fusobacteria, and Firmicutes). A primer set that amplified the fumarate reductase gene ( frdA) from Proteobacteria was developed and two frdA clone libraries were constructed. Identities of deduced amino acid sequences of cloned frdA amplicons against known sequences ranged from 58% to 85% suggesting the presence of unknown fumarate reducing bacteria. This is the first report on the diversity of fumarate reducing bacteria in the rumen.
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