Diversity of Epiphytic Subaerial Algal Communities in Bangkok, Thailand, and Their Potential Bioindicator with Air Pollution

Abstract

Epiphytic subaerial algae represent an assemblage of micro-organisms widely distributed in terrestrial environments, including urban environments. Urban habitats present many challenges for the survival of photosynthetic micro-organisms, yet many species of subaerial microalgae have been reported from these environments, demonstrating a high tolerance to the harsh conditions of urban environments. In this study, the epiphytic subaerial communities of five parks in the urban area of Bangkok were studied using a metabarcoding approach (sequencing of the 23S rDNA marker), with the goal of unraveling their diversity and assessing potential bioindicators with levels of air pollution. Diversity indexes were determined for the algal taxa detected, which were separated into groups corresponding to different collection sites by cluster analysis. Relationships between taxa and air pollutants were analyzed by PCA and the Pearson correlation coefficient (r). The results showed a high diversity of epiphytic subaerial algae. We recorded 101 taxa belonging to the Cyanophyta (70 taxa), Chlorophyta (21 taxa), Charophyta (5 taxa), Bacillariophyta (3 taxa), and Eustigmatophyta (2 taxa). The most abundant taxon was Chroococcidiopsis sp. 1, for which up to 13,254 sequences/cm2 were recorded. The Shannon–Weaver index ranged between 1.37 and 2.51, the Margalef index between 3.84 and 4.75, and the Pielou index between 0.30 and 0.54. The similarity index was between 8.00% and 64.82%, according to the cluster analysis results for the three groups. The PCA indicated that all air pollutants affected the diversity and abundance of epiphytic subaerial algae. Cyanothece sp. 2 was negatively related to O3 and positively related to NO2 and CO and is suggested as a potential bioindicator of air pollution.