Abstract
In order to clarify the composition of endophytic fungi in Gentiana rhodantha in colonization tissues with four Liupanshui sampling sites (Fenghuang Zhongshan, Nankai Shuicheng, Yangmei Shuicheng and Xinchang Liuzhi) in Liupanshui, strains were isolated with health leaf, stem and root and identification based on morphological characteristics and ITS sequence analysis. As well as screening for biocontrol stains by the dural culture method on PDA plates with Sclerotinia sclerotionrum . The results showed that G. rhodantha concealed rich fungal diversity, the colonization rate and isolation rate of endophytic fungi reached 49.38% and 61.25% respectively. 294 strains of endophytic fungi were obtained from 480 explant tissue segments of G. rhodantha and belonging to 2 phylum, 5 classes, 14 orders and 32 genera; of which Fusarium and Colletotrichum were comparatively advantageous taxa in endophytic fungi of G. rhodantha , and the species contained occupied 9.86% and 8.84% of the total endophytes respectively．According to the calculation results, Xinchang LZ in four sites and stem in three tissues showed optimum degrees of diversity, evenness and dominance respectively. 23 strains of endophytic fungi with inhibitory activities against S. sclerotionrum by the dural culture method; Amomg them, the inhibition rate of Botryosphaeria sp. R022, R021, Phomopsis sp. S002, Colletotrichum sp. S028 and Fusarium sp. L031 were higher than 70% and showed the best inhibitory potential.
Highlights
Endophytic fungi have been isolated in all plant tissues for parts of their life cycle, and not causing obvious symptoms (Wemheuer et al, 2019)
294 fungal strains were obtained in 480 health G. rhodantha tissues fragments by tissue isolation method in 4 collection sites of Liupanshui city, Guizhou, China
Colonization rate and isolation rate of Fenghuang, ZS were higher than another three sites, as well as two indexes in root are higher than leaf and stem (Table 1)
Summary
Endophytic fungi have been isolated in all plant tissues for parts of their life cycle, and not causing obvious symptoms (Wemheuer et al, 2019). Endophytes often form a mutualistic interactions with their host, with the relationship benefitting both partners (Deshmukh et al, 2006). The relationships between endophytic fungi and medicinal plants have more reported in recent years. Guo et al (2000) isolated 25 endophytic strains in Dendrobium officinale, and belonging to 17 genera. Ming et al (2010) obtained 48 fungal strains in root of ginseng, and they are belonging to 8 family, 9 genera and 12 species by ITS sequences date analysis. Zhang et al (2019) have shown that group compositions and isolated rate of endophytes were volatile with different collected sites, isolated tissues and seasons
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