Diversity of endophytic bacteria producing an antibacterial against colorectal bacteria from velvet apple (Diospyros blancoi A.DC.) fruit and stem

Abstract

In this study, an exploration of endophytic bacteria producing antibacterial against colorectal bacteria from velvet apple fruit and stem was carried out. Velvet apple (Diospyros blancoi A.DC.) is a plant used as a traditional medicine because of its ability to inhibit the growth of bacteria. This study aims to obtain information on extracts and isolates of endophytic bacteria from velvet apple fruit and stem that have the greatest potential to inhibit the growth of colorectal bacteria Salmonella enterica and Enterococcus faecalis. The method used is the descriptive exploratory method through the stages of isolation and identification of endophytic bacteria, extraction of velvet apple fruit and stem, antibacterial activity test, and identification of bioactive compounds. The results showed that extracts and endophytic bacteria of velvet apple fruit and stem could inhibit the growth of both types of colorectal bacteria with moderate to very strong and moderate to strong ability. Identification of endophytic bacteria showed potential isolates of endophytic bacteria were Bacillus cereus, Bacillus megaterium, Brevibacillus choshinensis, and Bacillus sp.