Diversity of Endophytic Bacteria in Parasitic and Non-Parasitic Centranthera grandiflora Benth. Based on High-Throughput Sequencing

Abstract

The community of endophytic bacteria varies with the genotypes, nutritional status and growth environment of host plants, and in turn it also affects the growth, metabolism and biochemical synthesis of plants. Using the root, stem and leaf of parasitic and non-parasitic Centranthera grandiflora Benth. (PR, PS, PL and NR, NS, NL) as materials, the differences of endophytic bacteria community and diversity were analyzed by high-throughput sequencing. Alpha diversity analysis shows that the richness and diversity of endophytic bacteria in NR were lower than those in PR; while those in NS were higher than those in PS. At the phylum level, Proteobacteria was the dominant phyla, followed by Bacteroidetes and Actinobacteria; the relative abundance of Proteobacteria in each tissue of non-parasitic sample (NR 93.87%, NS 84.27%, NL 90.00%) was higher than that in parasitic sample (PR 77.06%, PS 78.28%, PL 84.96%). The relative abundance in roots increased the most, up to 16.84%. At the genus level, each sample had its own dominant group, Acinetobacter (37.90%) was the dominant in NR, and the relative abundance was 9.56 times than that in PR. The nutritional status and the endophytic bacteria community of host plants have an impact on the structure and diversity of endophytic bacteria in Centranthera grandiflora Benth.; the nutrients needed for Centranthera grandiflora Benth. growth may not only come from the host plant, but also from endophytic bacteria. The results aim to provide a theoretical basis for related study in the relationship between root hemiparasitic plants and their hosts.