Diversity of edible canna (Canna edulis Ker.) in Surakarta region based on the morphological characters and isozym band pattern.

Abstract

Ashary SS, Etikawati N, Suratman. 2016. Diversity of edible canna (Canna edulis Ker.) in Surakarta region based on the morphological characters and isozym band pattern. Bioteknologi 13: 18-33. The aim of the research is to study the diversity and relationship of Edible Canna in Surakarta region based on the morphological characters and isozym band pattern. This research included morphological observation of Edible Canna and electrophoresis of the rhizomes to get isozym band pattern. Edible Canna’s fresh rhizomes were extracted with extract buffer and then centrifuged at 13000 rpm during 20 minutes. Supernatan and loading dye were mixed and then electrophored with poliacrilamide gel. The morphological characters and isozym band pattern were analysed using Similarity Index (SI) and computed with Numerical Taxonomy and Multivariate Analysis System (NTSYS) program 2.0 version until get the dendogram. From the research, it can be concluded that there were diversity of Edible Canna based on the morphological characters and isozym band pattern. The diversity of morphological ganyong included the colour and size of Edible Canna’s organs. The diversity of isozym band pattern included emergence and thickness of band. Based on the morphological characters and isozym band pattern, Edible Canna from Wonogiri and ganyong from Sukoharjo have the closest relationship with similarity coefficient 48%, whereas the farthest was the relationship of C. hybrida with the others at similarity coefficient of 11.57%.