Abstract
During a survey conducted in autumn 2022 and spring 2023, extensive leaf spots were observed on Aristolochia grandiflora plants in the Botanical Garden “Angelo Rambelli” (Viterbo). To preserve the botanical garden collection and avoid disease spread, morphological and molecular identification of the causal agent were carried out. The results revealed three distinct Colletotrichum species belonging to the Colletotrichum boninense and Colletotrichum orchidearum species complexes, which have never been reported together within the same host and, for two of them, never in Italy. These findings may contribute to further extend the state of the art on leaf anthracnose, as well as provide new insights and molecular data for further phylogenetic studies.
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