Diversity of allochtonous substances detected in bee pollen pellets

Abstract

Received: 2016-08-07 | Accepted: 2016-09-13 | Available online: 2017-09-30 http://dx.doi.org/10.15414/afz.2017.20.03.60-65 This paper quantifies the diversity of natural and artificial allochthonous materials in bee pollen pellets and assesses their impact on potential applications. Bee products used in medicine, pharmacology and food products contain honey bee wax, propolis and flower pollens, and bee pollen pellet composition is dependent on the flower’s locality and methods used in technological preparation and storage. The quality of commercially available pollen and its positive and negative mode-of-actions are significantly influenced by natural and artificial allochthonous substances. The flower pollen pellets for this study were obtained from the Levice district in the Slovak Republic and analysed by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS). These visual and chemical analyses confirmed; (i) 4 different botanical pollen species were present in the pellets, (ii) minimal harmful substances were detected; with bee fragments and dead fungal hyphae biomass noted, (iii) different types of soil particles/aggregates were adsorbed; mainly Fe, Si oxides, silicates and alumosilicates and (iv) analysis revealed one artificial Ti-Mn-Fe grain, but this was most likely a residue from technological processes. Determination of all hazardous substances is necessary for bee pollen to be widely commercially available as food nutritional and energy supplements, and this can be achieved by microscopic study and the wide range of current analytical techniques. Keywords:  bee pollen, food sources, pollen pellets, soil particles, artificial contaminants References Almeida-Muradian, L.B. et al. (2005) Chemical composition and botanical evaluation of dried bee pollen pellets. In Journal of Food Composition and Analysis , vol. 18, pp. 105−111. Campos, M.G.R. et al. (2008) Pollen composition and standardisation of analytical methods. In Journal of Apicultural Research , vol. 47, no. 2, pp. 154−161. Chauzat, M.P. et al. (2006) A Survey of Pesticide Residues in Pollen Loads Collected by Honey Bees in France. In Journal of Economic Entomology , vol. 99, no. 2, pp. 253−262. CURLIK, J. (2011) Potentially toxic microelements and their distribution in soils of Slovakia. Bratislava: Suma print (in Slovak). de Oliveira, R. C. et al. (2016) Bee pollen as a bioindicator of environmental pesticide contamination. In Chemosphere , vol. 163, pp. 525−534. Estevinho, L.M. et al. (2012) Portuguese bee pollen: Palynological study, nutritional and microbiological evaluation. In International Journal of Food Science and Technology , vol. 47, pp. 429−435. Futak, J. (1984) Phytogeographical division of Slovakia. In Flora Slovenska IV/1 . Bratislava: Veda, pp. 418−419 (in Slovak). Hooda, P.S. et al. (2004) The potential impact of soil ingestion on human mineral nutrition. In Science of The Total Environment , vol. 333, pp. 75−87. Kacaniova, M. et al. (2004) Microflora of the honeybee gastrointestinal tract. In Folia Microbiologica , vol., 49, no. 2, pp. 169−171. Kacaniova, M. et al. (2011) Mycobiota and mycotoxins in bee pollen collected from different areas of Slovakia. In Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes , vol. 46, pp. 623−629. Kim, H., Suh, D.W. and Kim, N.J. (2013) Fe-Al-Mn-C lightweight structural alloys: A review on the microstructures and mechanical properties. In Science and Technology of Advanced Materials , vol. 14, pp. 1−12. Klimko, M., Kluza, M. and Kreft, A. (2000) Morphology of pollen grains in three varieties of Helianthus annuus L. In Roczniki Akademii Rolniczej w Poznaniu CCCXXII Botanika , vol. 3, pp. 135−142. Knox, R.B. et al. (1997) Major grass pollen allergen Lol p 1 binds to diesel axhaust particles: Implications for asthma and air pollution. In Clinical and Experimental Allergy , vol. 27, pp. 246−251. Konvickova, Z. et al. (2016) Antimicrobial bionanocomposite–from precursors to the functional material in one simple step. In Journal of nanoparticle research, vol. 18, pp. 368. Kořenkova, L. et al. (2017) Physiological response of culture media-grown barley (Hordeum vulgare L.) to titanium oxide nanoparticles. In Acta Agriculturae Scandinavica Section B: Soil and Plant Science , vol. 67, pp. 285−291. Lin, H., Gomez, I. and Meredith, J.C. (2013) Pollenkitt wetting mechanism enables species-specific tunable pollen adhesion. In Langmuir , vol. 29, pp. 3012−3023. Linskens, H.F. and Jorde, W. (1997) Pollen as food and medicine - A review. In Economic Botany , vol. 51, no. 1, pp. 78−86. Margaoan, R. et al. (2010) Bee collected pollen–General aspects and chemical composition. In Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies , vol. 67, no. (1-2), pp. 254 −259. Miklos, L. et al. (2002) Landscape atlas of the Slovak Republic . Bratislava: Ministerstvo životneho prostredia SR. Nogueira, C. et al. (2012) Commercial bee pollen with different geographical origins: A comprehensive approach. In International Journal of Molecular Sciences , vol. 13, pp. 11173−11187. Ormstad, H., Johansen, B.V. and Gaarder, P.I. (1998) Airborne house dust particles and diesel exhaust particles as allergen carriers. In Clinical and Experimental Allergy , vol. 28, pp. 702−708. Punt, W. et al. (2007) Glossary of pollen and spore terminology. In Review of Palaeobotany and Palynology , vol. 143, pp. 1−81. Ruby, M.V. et al. (1999) Advances in evaluating the oral bioavailability of inorganics in soil for use in human health risk assessment. In Environmental Science and Technology , vol. 33, pp. 3697−3705. Schulte, F. et al. (2008) Chemical characterization and classification of pollen. In Analytical Chemistry , vol. 80, pp. 9551−9556. Schulz, S. et al. (2000) Composition of lipids from sunflower pollen ( Helianthus annuus ). In Phytochemistry , vol. 54, pp. 325−336. STRBA, P. and KOSAR, G. (2012) Diversity of vascular plants in agricultural landscape of central part of Žitný ostrov region. In Biodiversity in agricultural landscape and ecosystem. International conference of the project REVERSE-INTERREG IVC. Piesťany: 13th of June 2012. Piesťany: Centre of Plant Production Piesťany, pp. 13−16 (in Slovak). Villanueva, M.T.O. et al. (2002) The importance of bee-collected pollen in the diet: A study of its composition. In International Journal of Food Sciences and Nutrition , vol. 53, pp. 217−224.