Abstract
Dendrobium sylvanum orchid is categorized as lowland habitus orchid and able to survive wihtout any shade. Orchid cultivation takes a very long time so it needs faster cultivation and producing large quantities. Therefore, it is necessary to do orchid culture by using tissue culture processes and then given gamma ray irradiation to bring up new characters in the orchid .The purpose of this research was to discover the impact of gamma ray irradiation in vitro to the diversity of D. sylvanum. The doses of gamma ray used in this research were 0 Gy, 15 Gy, 30 Gy, 45 Gy, and 60 Gy. The results obtained indicated that mutation mostly occurred on the treatment with 15 Gy (4.4 cm of plant height, 2.67 of leaves, 2.36 cm of leaf length, 0.49 cm of leaf width, and 5.33 of root strands) compared to the other doses and the control plants. The new finding in this research is the gamma ray dose that can optimally stimulate the mutation in D. sylvanum. This information is useful to generate the new variety in orchid cultivation in Indonesia. This research provides innovation in orchid cultivation and new variety that is possible to arise after the mutation.
Highlights
Dendrobium is one of the genera of orchid plants which becomes the favorite in the Indonesian community and the second most favorite in the world
The purpose of this research was to discover the impact of gamma ray irradiation in vitro to the diversity of D. sylvanum
The acclimatization stage caused stress on the plant treated by gamma ray irradiation
Summary
Dendrobium is one of the genera of orchid plants which becomes the favorite in the Indonesian community and the second most favorite in the world. Dendrobium is the most favorite commodity because the nature of this flower is relatively more sustained compared to the other types of orchid and it has variation of colors, which make its market potential bigger. In the regions of Borneo, there are 143 species of Dendrobium orchid and most of them are discovered in the forests (Sabran et al, 2003). Dendrobium orchid could be found in Eastern part of Indonesia, such as Papua and Maluku (Widiastoety et al, 2010). One of the species of Dendrobium which becomes the favorite is Dendrobium sylvanum. D. sylvanum orchid could only be found in the areas of Papua New Guinea, New Ireland, Bougainville, and Solomon Island. The flower is relatively last longer and in one blooming period, it could produce about 30-40 of flower buds
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