Diversity and Heavy Metal Tolerance of Fungi Associated with Different Coal Overburden Strata of Tikak Colliery, Assam.

Abstract

Coal mine overburdens are generally highly acidic and contaminated with toxic heavy metals. Here, we studied the culturable fungal diversity associated with different coal overburden strata (OBS) of Assam, India, and assessed their heavy metal tolerance ability against five different heavy metals viz., As3+, Cd2+, Cr6+, Cu2+, and Ni2+. Among 15 distinct coal OBS considered in this study which spans a depth of ~ 35m from the ground surface, the isolation of fungi was successful only from 11 OBS samples and the colony-forming unit (CFU) counts were highly variable among the samples. A total of 66 fungal pure cultures were isolated which belong to 18 genera (17 known and 1 unknown) under 15 families and two divisions i.e., Ascomycota (89.4%) and Basidiomycota (10.6%). Acidiella bohemica was found relatively the most abundant species followed by Rhodotorula toruloides. A good number of fungal isolates was found tolerant to the test heavy metals at concentrations ≥ 1mM. Findings of some multi-metallotolerant fungal isolates along with a tolerance up to 5mM concentration of As3+, and up to 10mM each of Cu2+, Cr6+, Ni2+ and Cd2+ were noteworthy in the present study that could be useful in the management of heavy metal pollution or stress. Cultivable fungal diversity of coal mine overburden strata of Tikak colliery, Margherita, Assam, India. It shows a photograph of the coal mining site as the background, front view of the fungal colonies in the upper section, and a graphical representation of heavy metal tolerance of the isolates at different concentrations of As, Cd, Cr, Cu, and Ni in the lower section.