Diversity and enzymatic potential of bacteria isolated from household waste compost

Abstract

The ecosystem in organic waste processing in Surabaya provides a resource of potential microorganisms in the form of bacterial isolates that degrade organic waste components. This study aims to isolate, test the enzymatic potential, and identify bacteria from urban organic waste in degrading the main components of organic waste. Bacteria were isolated from compost samples obtained from household organic waste processing in Surabaya. The compost sample was mixed with sterile physiological water then the suspension is inoculated on nutrient agar media. The compost indigenous bacterial isolates obtained were then characterized macroscopically and microscopically. All isolates obtained were then tested for their potential in producing amylase, cellulase, protease, and lipase enzymes on agar selective media. A total of 12 compost indigenous bacteria that were successfully isolated showed different macroscopic, microscopic and enzymatic potential characters. The three most potent bacterial isolates that can hydrolyze organic waste components from starch, cellulose, protein, and lipid compounds are KO A8, KO A9, and KO A11. Two isolates KO A8 and KO A9 belong to Bacillus, and one isolates code KO A11 belongs to Actinobacillus. Compost-indigenous bacteria showed the potential to be applied as a microbial formula in the processing of household organic and industrial wastes to help zero waste environment.