Abstract
A 30-year fertilizer experiment was conducted to investigate the effects of organic manure and mineral NPK fertilizer on black soil. Four experimental treatments were used: CK, organic manure, mineral NPK fertilizer, and organic manure plus mineral fertilizer. The diversity of the nirS gene was analyzed by PCR-restriction fragment length polymorphism. The Shannon diversity index and Evenness factor, Simpson’s diversity and Shannon-Weiner indices, principal component analysis and a one-way analysis of similarities test showed that organic manure made the opposite contribution to soil diversity of nirS genes compared to mineral fertilizer treatment, which resulted in increased diversity of the nirS gene. Comparison of the phylogenetic analysis of nirS genes in the mineral fertilizer and organic manure clone libraries showed that induction by mineral fertilizer was stronger than that by organic manure, based on the types of unique nirS genotypes found in the two libraries and the evolutionary distances between them.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
