Abstract
Several barley yellow dwarf virus (BYDV) isolates collected from viruliferous aphids in upstate New York were identified as PAV serotypes, based on their reaction with a polyclonal antiserum to NY-PAV. Four of six isolates examined were distinguished from the NY-PAV type isolate of BYDV by their failure to react with a PAV-specific monoclonal antibody in enzyme-linked immunosorbent assay (ELISA) and by restriction fragment length polymorphisms of polymerase chain reaction-amplified viral sequences. The capsid protein amino acid sequence of one of these four isolates, designated PAV-129, was less similar to that of NY-PAV (86.5% similar) than NY-PAV is to two other isolates, serotyped as PAV, from Indiana (98% similar) or Australia (97% similar). In biological comparisons of PAV-129 and NY-PAV, the efficiency of transmission by two aphid species, Rhopalosiphum padi and Sitobion avenae, was not significantly different; however, PAV-129 caused more severe symptoms when inoculated to a variety of oat genotypes. In addition, the growth and grain yield of 'Ogle', a spring oat considered resistant to the BYDV PAV serotype was significantly reduced when infected with PAV-129.
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