Abstract
A newly isolated Cryptococcus laurentii was assessed for endo-xylanase production by utilizing different sources of nitrogen in two concentrations. The highest xylanase production was recorded when used were ammonium nitrate (6.2 U.mL−1) and ammonium sulfate (6.0 U.mL−1) at 3% concentration. An experimental design CCRD 22 was conducted, in which the effect of the variables xylan and yeast extract over the endo-xylanase activity were evaluated. The conditions to maximize the production (14 U.mL−1) of target enzyme were xylan concentration of 18.6 gL−1, 8.7 gL−1 of yeast extract, 3.0 gL−1 of ammonium sulfate, pH 6.5 agitation of 175 rpm and 30 °C, using these settings a low level of β-xylosidase (0.006 U.mL−1) it is found, which ensures that this enzyme several industrial applications. Through this study, it was possible to maximize the endo xylanase activity produced by C. laurentti using the experimental planning tool, the increase in xylanolytic activity was around 400%.
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