Abstract
Latelly, aquiculture production in Brazil has made great strides. Among the native species cultivated in Brazil, piapara (Leporinus elogatus) has been widely praised. With the objective of evaluating restocking programs, the variability and genetic divergence of three piapara stocks were analyzed using the RAPD (Random Amplified Polymorphic DNA) technique. The first stock belongs to the Aquiculture and Hydrology Station of Duke Energy International (A); the second one belongs to a fish farm in the city of Rolândia (B); and the third to the River Restocking Program of Paraná (C). The ten primers used for RAPD produced 105 polymorphic loci, conferring a polymorphism of 98.1% for the three evaluated stocks. Polymorphic loci percentage and Shannon index were higher for stock A. However, all values were high, indicating high intrapopulation diversity. The Gst values indicate a low genetic variation between A x B stocks and a moderate differentiation among the others. The Nm was higher between A x B stocks. Genetic distance and the dendrogram indicate that A x B stocks are genetically less distant.
Highlights
A produção aquícola brasileira passou de 20,5 mil toneladas, em 1990, para 210 mil toneladas, em 2001, com um aumento de 925%, enquanto a aquicultura mundial teve um crescimento de 187% no mesmo período, o que fez com que o país ocupasse a 19a posição na produção de organismos aquáticos (Borghetti et al, 2003)
Among the native species cultivated in Brazil
The first stock belongs to the Aquiculture
Summary
Para avaliar a variabilidade e a divergência genética, foram utilizadas três populações estocadas de piapara (Leporinus elongatus); a primeira pertencente à Estação de Aquicultura e Hidrologia da Duke Energy International (Geração Paranapanema) (A), localizada no município de Salto Grande, Estado de São Paulo; a segunda pertencente à pisicultura de Rolândia, Estado do Paraná (B); e a terceira pertencente ao Programa de Repovoamento dos rios do Paraná (C). Foram utilizadas 57 amostras de reprodutores, sendo indivíduos pertencentes a Duke Energy, indivíduos à piscicultura de Rolândia e 27 amostras de larvas fornecidas pelo Programa de Repovoamento dos rios do Paraná. Estes estoques de piapara são destinados à produção de alevinos para o programa de repovoamento dos rios do Paraná, financiado pelo Governo do Estado do Paraná
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