Diverse T-cell profiles in children with type 1 diabetes and/or celiac disease

Abstract

Event Abstract Back to Event Diverse T-cell profiles in children with type 1 diabetes and/or celiac disease Maria Faresjö1*, Karin Åkesson2, Anna Kivling3 and Anna Rydén3 1 School of Health Sciences, Jönköping University, Department of Natural Science and Biomedicine, Sweden 2 Ryhov County Hospital, Clinic of Pediatrics, Sweden 3 Faculty of Health Sciences, Linköping University, Department of Clinical and Experimental Medicine, Sweden Type 1 diabetes (T1D) and celiac disease (CD) are both characterized by an autoimmune outcome. As T1D and CD share the same risk genes, patients have risk of developing the other disease subsequently. This study aimed to investigate the expression of T-helper (Th)-, T-cytotoxic (Tc)- and T-regulatory (Treg) cells in children with T1D and/or CD in comparison to healthy children. Subgroups of T-cells (CD4+ or CD8+); naïve (CD27+CD28+CD45RA+CCR7+), central memory (CD27+CD28+CD45RA-CCR7+), effector memory (early differentiated;CD27+CD28+CD45RA-CCR7- and late differentiated;CD27-CD28-CD45RA-CCR7-), terminally differentiated effector cells (TEMRA;CD27-CD28-CD45RA+CCR7-) and Treg (CD4+CD25+FOXP3+CD127-) cells, and their expression of CD39, CD45RA, CD101 and CD129, were studied by flow cytometry in children with T1D and/or CD or without any of these diseases (references). Children with exclusively T1D had higher MFI of FOXP3 (p<0.05) but lower percentage of CD39+ and CD45RA+ within the Treg population (CD4+CD25+FOXP3+CD127-) (p<0.05). Children with exclusively CD had a higher MFI of CD101 (p<0.01) as well as a higher percentage of CD129+ (p<0.05), in the CD4+CD25hi lymphocyte population, compared to references. Children diagnosed with both T1D and CD showed higher percentage of terminally differentiated (TEMRA) CD4+ cells (p<0.05), but lower percentages of effector, both early and late, memory CD8+ cells (p<0.05), compared to references. In conclusion, children with combined T1D and CD have higher percentage of differentiated Th-cells, compared to Tc-cells. T1D children show signs of low CD39+ Treg cells that may indicate loss of suppressive function. On the contrary, CD children show signs of CD101+ Treg cells that may indicate suppressor activity. Acknowledgements Medical Research Council of South-East Sweden (FORSS) Keywords: T-helper cells, T-cytotoxic cells, T-regulatory cells, type 1 diabetes, Celiac Disease Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Faresjö M, Åkesson K, Kivling A and Rydén A (2013). Diverse T-cell profiles in children with type 1 diabetes and/or celiac disease. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00369 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 19 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Prof. Maria Faresjö, School of Health Sciences, Jönköping University, Department of Natural Science and Biomedicine, Jönköping, Sweden, maria.faresjo@hhj.hj.se Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Maria Faresjö Karin Åkesson Anna Kivling Anna Rydén Google Maria Faresjö Karin Åkesson Anna Kivling Anna Rydén Google Scholar Maria Faresjö Karin Åkesson Anna Kivling Anna Rydén PubMed Maria Faresjö Karin Åkesson Anna Kivling Anna Rydén Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.