Diverse responses of tolerant and sensitive lines of Chickpea to drought stress

Abstract

Drought is a worldwide concern and designation of drought stress adaptive mechanisms is one of the main directions in plant physiology and crop breeding. Genotypes diversity can be used to identify effective unexploited genes and pathways. In order to that, the effect of varying terminal drought intensity treatments on physiological and biochemical traits was evaluated in ILC3279, ICCV2 and FLIP9855 C chickpea lines. Well-watered, intermediate and severe drought treatments were applied from flowering till maturity. Photosynthetic efficiency, membrane stability, soluble sugar and proline content, leaf protein profile, and antioxidant enzyme activities were compared on 1st, 3rd, and 5th week after applying stress. Based on the results, it was found that the susceptibility of photosynthetic machinery of ILC3279 was more than others. Tolerant genotypes responded to drought differently; an increase and a decrease in catalase activity have been observed in ICCV2 and FLIP9855 C, respectively. The prominent role of soluble sugars was observed in ICCV2. Expressions of polypeptides 27 and 45 kDa in tolerant lines refer to their possible role in drought stress adaptation. Generally, in spite of significant variability in chickpea lines to cope with drought, lower ascorbate peroxidase activity, higher peroxidase activity, and higher Fv/Fm ratio can be tested as markers of chickpea drought tolerant.