Abstract
The regulatory function of sucrose in the activity of lipid-degrading enzymes was investigated in germinating seeds of yellow lupin (Lupinus luteus L.), white lupin (Lupinus albus L.) and Andean lupin (Lupinus mutabilis Sweet). The study was conducted on isolated embryo axes, excised cotyledons and seedlings cultured in vitro for 96 h on medium with 60 mM sucrose or without the sugar. The activity of lipase (lipolysis), acyl-CoA oxidase and catalase (fatty acid β-oxidation) was enhanced in all studied organs cultured on medium without sucrose. The activity of cytosolic aconitase (glyoxylate cycle) was stimulated by sucrose in seedling axes and isolated embryo axes, whereas in seedling cotyledons and excised cotyledons, it was inhibited. The regulatory function of sucrose in phosphoenolpyruvate carboxykinase (gluconeogenesis) was observed only in isolated embryo axes and the activity was lower in carbohydrate deficiency conditions. The peculiar features of storage lipid breakdown in germinating lupin seeds and its regulation by sucrose are discussed.
Highlights
Lupin seeds differ significantly in storage lipid content
The activity of lipase, acylCoA oxidase and catalase was enhanced in all studied organs cultured on medium without sucrose
The regulatory function of sucrose in activity of enzymes involved in storage lipid breakdown during seed germination of yellow, white and Andean lupin was investigated
Summary
Lupin seeds differ significantly in storage lipid content. The clearest example of a high lipid level in mature seeds is Andean lupin (Lupinus mutabilis Sweet), which accumulates up to 20 % of lipid in seed dry matter. Lipid content in seeds of this species is about 6 %. White lupin seeds (Lupinus albus L.) contain 7–14 % (Borek et al 2009, 2012a). Andean lupin seeds are similar to soybean seeds, which contain 12–26 % of storage lipid (Zhou et al 2006). Apart from storage lipid, lupin seeds contain large quantities of protein (up to 40–50 %), which is the dominant storage compound in these species (Santos et al 1997; Duranti et al 2008; Borek et al 2012b)
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