Divergent selection for uterine capacity in rabbits. III. Responses in uterine capacity and its components estimated with a cryopreserved control population1

Abstract

This work evaluated the response to 10 generations of divergent selection for uterine capacity (UC) in rabbits to determine whether this response was symmetric by contrasting both lines against a cryopreserved control population. Animals came from the 13th generation of an experiment of divergent selection for UC and from a cryopreserved control population. The two UC lines were divergently selected for 10 generations, and selection was relaxed from the 11th generation until the 13th generation. Uterine capacity was estimated as litter size (LS) in unilaterally ovariectomized (ULO) does. To create the control population, embryos from the base generation were vitrified and stored in liquid N2 for 10 generations. Data from 461 pregnancies produced by 134 ULO does were used: 62 does from the high UC line, 55 females from the low UC line, and 17 females from the control line. The following traits were analyzed: ovulation rate (OR); number of implanted embryos (IE); (UC), estimated as total number of rabbits born; number born alive (NBA); prenatal survival (PS), estimated as UC/OR; embryo survival (ES), estimated as IE/OR; and fetal survival (FS), estimated as UC/IE. Ovulation rate, IE, PS, ES, and FS were measured by laparoscopy only in the second parity. Uterine capacity and NBA were measured over four parities. Responses in UC and its components were estimated as differences between the selected lines and the control line using a Bayesian approach. Selection for UC led to differences of 1.01 kits between the high and low lines, but this response was asymmetric. No differences were found between the high and control lines (high - control = -0.08), whereas the low and control lines differed by 1.08 kits, with a probability of the difference being greater than zero of 0.98. Difference between the high and low lines and between the control and low lines was one-half of the difference reported for correlated response in LS in previous studies. No differences in OR were detected among lines. The control and low lines differed by 1.06 IE, with a probability of the difference being higher than zero of 0.84. Prenatal survival for the low line was less than that of the control line. In summary, selection for UC was asymmetric, which was mainly due to a correlated response in PS. Response in UC was one-half of the difference reported for correlated response in LS in previous studies.