Divergences in learning and memory among wild zebrafish: Do sex and body size play a role?

Abstract

Given its diverse ecological distribution, zebrafish has great potential for investigations on the effect of habitat characteristics on cognition. Studies were conducted on four wild-caught zebrafish populations to understand the role of native habitat, sex, and body size in determining learning through a novel task associated with a food reward. The habitat variables, namely, the relative abundances of zebrafish and predatory fish and the substrate and vegetation diversity, were quantified during fish sampling. Fish were subjected to a novel task to find a food reward in a maze over successive training trials followed by a test for memory. Performances of subjects were based on time taken to find the food reward and number of mistakes made during trials, and tests for memory. The experiments revealed significant differences in learning rates and memory across populations. Males made significantly fewer mistakes than females only within two populations. No relationship between performance and body size was observed. The differences in learning and memory among wild zebrafish could be due to differences in predation, complexity, and stability of the native habitats. These findings suggest the possible role of multiple interacting factors in determining learning and memory among populations and point to a need for incorporating effects of several factors in future studies.