Diurnal variations in the kinetics of delayed luminescence from Scenedesmus obtusiusculus after exposure to various light and CO2 regimes

Abstract

Delayed luminescence was measured from samples of a synchronously growing cell culture of the unicellular green alga, Scenedesmus obtusiusculus Chod., every second hour during the 24 h cell cycle under a 15/9 h lighi/dark regime. Both high (air + 2.5% CO2) and low (0.03% CO2) CO2 conditions were used. Under high CO2 conditions, while light excitation induces formation of a late (maximum reached after 10–60 s) transient peak in delayed luminescence in cells sampled after 10–16 h in the cell cycle. During most of the cell cycle low CO2 conditions stimulate a late transient peak formation. Excitation with 700 nm light, but not with 680 nm light, induces a late transient peak in delayed luminescence under high CO2 conditions. The transient peak is more or less pronounced depending on the cell stage. The variations might be due to a changing capacity for light‐induced state I/stale II transitions during the cell cycle. It is assumed that the formation of a late transient peak in delayed luminescence is due to ATP hydrolyzation and is thus favoured by a high ATP/NADPH ratio. Hydrolyzation of ATP affects the transthylakoidal ΔpH, which regulates the reverse electron flow to the plastoquinone‐pool and QA/QB, thus affecting the decay kinetics of the delayed luminescence.