Abstract
Experiments on C57BL/6J mice showed that the mitotic index in the esophageal epithelium differs considerably in the morning and evening. The use of the colchamine (colcemid) method showed that the duration of mitosis in the morning and evening is about equal, namely 34–49 min (the results varied depending on the dose of colchamine and the duration of its action). Diurnal differences in the mitotic index of the esophageal epithelium thus depend on differences in mitotic activity (the number of mitoses in unit time). The need for observing several demands when the colchicine method is used, in order to obtain reliable results, is emphasized.
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