Abstract
Summary Six Jersey cows were bled six times daily at 4-hr, intervals to determine the diurnal variation in blood serum calcium. The variation between cows in serum calcium levels was significant, but daily and diurnal variations were not significant. Four Jersey cows were bled daily for 9 days and four times daily at 6-hr, intervals for 4 days during days four, five, six and seven of the 9-day period and blood serum citric acid and magnesium were determined. No significant difference existed between cows or between days in serum magnesium levels. However, a small but significant diurnal variation existed, with the low level being observed at the 9: 00 a.m. bleeding. Serum citric acid levels showed a significant variation between days and a highly significant variation between cows, but no significant diurnal variation. Therefore, under the conditions of the experiment, the time of day at which samples were taken had no significant effect upon serum calcium and citric acid levels. The blood serum magnesium was lowered at one bleeding.
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