Diurnal variation in the cellular and humoral immune responses of Japanese quail: Role of melatonin

Abstract

Experiments were conducted to determine if diurnal variations occur in the cellular and humoral immune responses of sexually mature, male Japanese quail and if this diurnal variation is mediated by the daily rhythm of melatonin. In Experiment 1, quail were exposed to LD 12:12 light–dark cycles and immune responses were measured in response to a single antigenic challenge given to different groups every 4 h over a 24 h period. Diurnal changes occurred in both the cellular and humoral immune responses. The cellular response was higher during the light phase than during the dark phase whereas the opposite was true for the humoral immune response. Experiment 2 was designed to determine if melatonin mediated these diurnal immune responses. Quail were maintained in continuous light (LL) to suppress endogenous melatonin production and half of them were given melatonin in the drinking water for 12 h each day for 2 weeks. Contrary to control quail, significant daily variations occurred in both the humoral and cellular immune responses of birds given melatonin. As in Experiment 1, the cellular and humoral immune responses were out of phase with one another, with the humoral response being maximal when melatonin was present. We may conclude that there exists a melatonin dependent diurnal variation in both cellular and humoral immune responses of quail. The responses were inverse to one another during the daily light–dark cycle with the cellular response being maximal during the daily light period and the humoral response being maximal during the daily dark period.