Abstract
BackgroundRecent clinical studies suggest that inflammatory mediators have huge potential in individualized therapy and in efficacy screening and can be utilized as biomarkers for a plethora of pathological conditions. The standard approach for detecting and measuring these inflammatory mediators is via blood samples. Nevertheless, there is no scientific report providing solid evidence on the most suitable blood compartment that will give the optimal inflammatory mediator measurement, or regarding the diurnal variation of circulating mediators. In this study, we present the biological variability of circulating cytokines and chemokines from healthy individuals (mean age 59 years) assessed by a novel membrane-based assay.MethodsFifteen males and an equal number of females (all above 50 years) with no known inflammatory condition were selected. Through a planar method, named Proteome Profiler™, improved with fluorescence readout into a semi-quantitative multiplex assay, a screening of 36 inflammatory mediators was performed in serum and plasma of morning and afternoon blood withdrawals.ResultsThe multiplex analysis revealed that the physiological variability of several circulating inflammatory mediators was relatively small within a cohort of 30 healthy aging subjects. There was no substantial gender effect in the inflammatory mediator profile. On the contrary, most of the cytokine/chemokine values measured in the afternoon collection were found to be higher compared to the morning ones, particularly in plasma.ConclusionsIn this study we provide evidence that circulating cytokine and chemokine levels of healthy individuals are elevated when blood is sampled in the afternoon compared to the morning, as influenced by the circulating cortisol levels. Furthermore, we report significant differences between cytokine/chemokine levels measured in serum and plasma. Our results provide essential information for future studies that will focus on examining circulating inflammatory mediator differences between healthy and diseased individuals.
Highlights
Recent clinical studies suggest that inflammatory mediators have huge potential in individualized therapy and in efficacy screening and can be utilized as biomarkers for a plethora of pathological conditions
Plasma collected in the afternoon contains higher concentrations of cytokines and chemokines than serum and plasma collected in the morning and serum sampled in the afternoon
The present data provide important information regarding the diurnal rhythm of cytokine/chemokine profile in serum and plasma in healthy aged individuals
Summary
Recent clinical studies suggest that inflammatory mediators have huge potential in individualized therapy and in efficacy screening and can be utilized as biomarkers for a plethora of pathological conditions. We present the biological variability of circulating cytokines and chemokines from healthy individuals (mean age 59 years) assessed by a novel membrane-based assay. Starting from a mere role of effector in the cell-mediated immune response, cytokines have been shown to be involved in aging-related diseases where they orchestrate the physio-pathological development of several diseases [2,3]. Evidence from both experimental and clinical trials indicates that inflammatory mediators are of paramount importance in the pathogenesis of chronic heart failure (HF), contributing to cardiac remodelling and peripheral vascular disturbances [4]. The role of the cytokines during the progression to HF is still unclear, several lines of evidence suggest that inflammatory mediators play beneficial as well as detrimental roles in the development of the pathology [5] and can predict the development and the outcome of HF
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