DIURNAL MODULATION OF PHOTOTROPIC RESPONSE BY TEMPERATURE AND LIGHT IN Chenopodium rubrum L. AS RELATED TO STEM EXTENSION RATE ANDARGININE DECARBOXYLASE ACTIVITY*

Abstract

Abstract— Seedlings of Chenopodium rubrum were grown under 12: 12 h light‐dark cycles with the light period at 32.5°C and darkness at 10°C (“normal” conditions) or with light at 10°C and darkness at 32.5CC (“inverse” conditions). When grown under unilateral white light with the photo‐thermoperiodic conditions described above, inverse seedlings did not show phototropic curvature while normal seedlings did. Regardless of temperature regime used, plants irradiated with unilateral blue light (456 nm) at 10°C failed to curve on subsequent incubation at 32.5°C. If the phototropic stimulus was given at 32.5°C followed again by 32.5°C in darkness, phototropic curvature was observed. The results with monochromatic blue light thus explain the lack of phototropic response of “inverse” seedlings with the unilateral light period given at 10°C. “Inverse “seedlings showed a sharp rhythm in their capacity to respond to phototropic stimulation at 32.5°C. The greatest capacity to respond came when the stem extension rate was actually zero. The differential growth accompanying phototropic curvature thus seems to be different from the growth responsible for uniform stem elongation. The curvature may result from differential turgor changes as in the case of sun tracking of leaves. “Inverse seedlings” showed clear signs of stress and displayed a rhythm in arginine decarboxylase, an enzyme thought to be stress‐related, with maxima during the low temperature light periods. It is concluded that the phototropic perception transduction chain is modified by the inverse conditions somewhere before the final growth‐turgor steps, since they exhibit geotropic sensitivity.