Diurnal influences on serum luteinizing hormone responses to opiate receptor blockade with naloxone or to luteinizing hormone-releasing hormone in the immature female rat.

Abstract

AbstractThe major objective of these studies was to determine whether there is a temporal pattern in the gonadotropin response of immature rats to luteinizing hormone-releasing hormone (LHRH) or the opiate antagonist, naloxone. Thirty-day-old female rats were injected at 3-hr intervals over a 24-hr period with either naloxone (2.5 mg/kg body wt) or LHRH (8 ng/100 g body wt). Animals were decapitated 15 min later and serum samples were assayed for luteinizing hormone (LH) by radioimmunoassay. The serum LH response to naloxone and LHRH varied significantly with the time of day. Naloxone administration had no statistically significant (P > 0.05) effect on levels of serum LH at 1500 and 1800 hr compared to levels in saline-injected controls, but induced a significant rise in serum LH at all other times. Naloxone had its greatest effect during the late evening and early morning hours (2100 to 0900 hr). A similar, but not identical, pattern of LH responsiveness to LHRH was observed, with the two rhythms being t...