Diurnal changes in activities of AspAT, A1AT, FDPA, MDH, ICDH and LDH in liver of mice fed high and low protein diet

Abstract

1. 1. Diurnal changes of the activities of AspAT, A1AT, FDPA, MDH, ICDH and LDH in liver of laboratory mice were studied. 2. 2. Experimental mice were fed granulated maize diet with a low (9%) protein content whereas control animals received the standard Murigran granulated diet with 25% protein content. 3. 3. The experiment was held for 7 weeks and animals were kept in L:D = 12:12 at 24 ± 2°C conditions. 4. 4. The liver samples were taken every 4 hours starting at 08.00 a.m. 5. 5. Diurnal rhythm in activity of AspAT, A1AT, MDH and LDH was observed in both groups while diurnal rhythm in activity of FDPA was found only in experimental animals. 6. 6. Diurnal rhythm in activity of ICDH was observed only in control mice. 7. 7. The low protein maize diet did not change the rhythmicity of enzymes studied, except the FDPA and ICDH activities.